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Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
 

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Article DOI: 10.3791/54017-v • 08:43 min • May 31st, 2016
May 31st, 2016 •
*1, *1,2, 3,4, 2, 1, 2, 1, 1, 1, 1,4, 1,4, 2,4, 1,2,4, 5, 1,4, 3,4, 1,2,4, 3,4, 1,5,6, 2,4
1Anesthesia Center for Critical Care Research of the Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, 2Cardiovascular Research Center and Cardiology Division of the Department of Medicine, Massachusetts General Hospital, 3Cardiovascular Division, Brigham and Women's Hospital, 4Harvard Medical School, 5Department of Anesthesiology, Uniklinik RWTH Aachen, RWTH Aachen University, 6Center for Immunology and Inflammatory Diseases and the Division of Rheumatology, Allergy, and Immunology of the Department of Medicine, Massachusetts General Hospital
* These authors contributed equally

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Vascular calcification is an important predictor of and contributor to human cardiovascular disease. This protocol describes methods for inducing calcification of cultured primary vascular smooth muscle cells and for quantifying calcification and macrophage burden in animal aortas using near-infrared fluorescence imaging.

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Calcification Vascular Smooth Muscle Cells Aortic Calcification Inflammation Imaging Ex Vivo In Vivo Primary Murine Aortic Smooth Muscle Cells Vascular Disease Biology Molecular Mechanisms Macrophage Activity Calcium Deposits Atherosclerotic Lesions Early States Of The Disease Procedure Tail Disinfection Tail Veins Syringe Needle Injection Calcium NIR Cathepsin NIR Aorta Harvest Dissecting Microscope
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