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An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis
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JoVE Revista Biología
An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis

An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis

DOI:
10.3791/54724-v

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09:17 min

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October 12, 2016

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1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Research Foundation

Capítulos

  • 00:05Título
  • 00:41Cell Fixation, Staining, and Pre-embedding
  • 03:54Osmification, Dehydration, and Embedding
  • 05:22Sectioning and Transmission Electron Microscopy
  • 07:32Results: Transmission Electron Analysis of Low Cell Number Samples
  • 08:37Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a protocol to prepare samples with low cell numbers for transmission electron microscopy (TEM) analysis.

Tags

Low Cell NumberTEM AnalysisSample PreparationHematopoietic Stem CellsFixationStainingAgarose EmbeddingCentrifugationReproducibilityQualitative And Quantitative Results

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