Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Rapid Isolation of BMPR-IB+ Adipose-Derived…
JoVE Journal
Developmental Biology

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Developmental Biology
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
 

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

Article DOI: 10.3791/55120-v • 09:07 min • February 24th, 2017
February 24th, 2017 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine

Capítulos

Resumen
Automatic Translation

Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

Tags

Rapid Isolation BMPR-IB+ Adipose-derived Stromal Cells Calvarial Defect Healing Model Osteogenic Activity Craniofacial Regenerative Biology Adipose-derived Stem Cells Fully Functional Bone Tissue Damaged Bone Defects Fresh Human Tissue Cell Culture Gene Expression Cell Service Marker Expression Large Bone Defects Cancer Trauma Infection Regenerative Fashion Visual Demonstration Calvarial Defect Creation Domed Skull Dura Mater Abdominal Fat Collection Flank Fat Collection Thigh Subcutaneous Fat Collection Sterile PBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter