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A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
 

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Article DOI: 10.3791/55425-v • 10:09 min • March 15th, 2017
March 15th, 2017 •
1,2, 1,2
1German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 2Institute of Ecology, Friedrich Schiller University Jena

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Resumen
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Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.

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Glucosinolate Extraction Analysis High-pressure Liquid Chromatography HPLC Plant-insect Ecology Plant Pathology Plant Breeding Food Sciences Well-validated Technique Wide Range Of Biological Samples Quantifying Glucosinolates Standard Lab Equipment G-25 Cross-linked Dextrin Gel Type H-1 Arylsulfatase Ultra-pure Water Absolute Ethanol Centrifugation
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