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Journal / Genetics / Chromatin Immunoprecipitation (ChIP) in Mouse…
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Genetics

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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
 

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Article DOI: 10.3791/55907-v • 11:39 min • June 17th, 2017
June 17th, 2017 •
1, 1, 1
1Institute of Biochemistry, University of Giessen

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This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.

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Chromatin Immunoprecipitation ChIP Mouse T-cell Lines Histone Marks Chromatin Biology T-cell Division T-cell Activation Chromatin Shearing Phenol-chloroform Isoamyl Alcohol Francesca Ferrante PhD Student Cell Culture Medium Formaldehyde Glycine PH 7.5 Centrifugation
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