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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
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JoVE Revista Biología del desarrollo
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

DOI:
10.3791/57785-v

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08:42 min

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June 26, 2018

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, , , , , ,
1Faculty of Biomedical and Health Sciences,Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research,Universidad Europea de Madrid

Capítulos

  • 00:04Título
  • 00:49β-galactosidase Histochemistry of Whole Mount Mouse Embryos
  • 02:23Paraffin Embedding of X-gal Stained Embryos
  • 04:37Paraffin Sectioning
  • 05:44Deparaffinization and Counterstaining
  • 06:56Results: X-gal Staining of E9.5 WT and Mt4-mmpLacZ/ Whole-mount Embryos and Tissue Sections
  • 07:52Conclusion

Summary

Traducción Automática

Traducción Automática

Here we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.

Tags

β-galactosidaseGene ExpressionWhole MountX-gal StainingMouse EmbryosBiología del desarrolloReporter GeneLineage TracingHistochemical DetectionParaffin Sectioning

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