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Cancer Research

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JoVE Journal Cancer Research
Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
 

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues

Article DOI: 10.3791/58551-v • 07:40 min • May 1st, 2019
May 1st, 2019 •
1, 2, 3, 1, 4, 4, 5, 6, 4, 4, 4, 1
1Department of Hematology, Zhongnan Hospital of Wuhan University, 2Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 3Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 4Department of Immunology, School of Basic Medical Sciences, Wuhan University, 5Department of Gastroenterology, Central Hospital of Wuhan, 6Department of Transfusion, Zhongnan Hospital of Wuhan University

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Resumen
Automatic Translation

ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results.

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Digital Analysis Immunostaining ZW10 Interacting Protein Human Lung Tissues Pathological Immunohistochemistry Technique Faster Digital Media High Quality Fixing Paraformaldehyde Dehydration Ethanol Immersions Xylene Immersion Paraffin Embedding Rotary Microtome Poly-L-lysine-coated Slides Dewaxing Dimethylbenzene Rehydration Antigen Repair Citric Acid Repair Liquid
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