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JoVE Journal Neuroscience
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
 

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Article DOI: 10.3791/59163-v • 09:41 min • March 20th, 2019
March 20th, 2019 •
1, 1, 1, 1, 1
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière

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Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.

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Preparation Immunostaining Myelinating Organotypic Cerebellar Slice Cultures Mechanism Of Myelination Remyelination Cellular Level Tissular Level Primary Cultures In Vivo Approaches Fast Model Accessible Model Tissue Architecture Melina Thetiot Remi Ronzano Postdoctoral Researcher PhD Student Foramen Magnum Skull Incision Dorsal Part Of The Skull Fine Forceps Brain Retrieval Optic Nerves Trigeminal Nerves Cell Culture Dish Dissection Medium Brain Orientation Binocular Microscope Hind Brain Separation Cerebellar Peduncles
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