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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
 

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Article DOI: 10.3791/60255-v • 07:14 min • September 20th, 2019
September 20th, 2019 •
*1, *1, 1
1Department of Biology and Biotechnology, Worcester Polytechnic Institute
* These authors contributed equally

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Here we present a protocol to assess the dynamics of spindle formation and mitotic progression. Our application of time-lapse imaging enables the user to identify cells at various stages of mitosis, track and identify mitotic defects, and analyze spindle dynamics and mitotic cell fate upon exposure to anti-mitotic drugs.

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Live Cell Imaging Metaphase Timing Cell Fate Mitotic Spindle Perturbations Dynamic Cellular Processes Cell Division Defects Daughter Cell Proliferation Viability Phototoxicity Optimization Aseptic Technique Biosafety Level Two Safety Cabinet Sterile Disposable Glass Pasteur Pipette Sterile PBS Trypsin Treatment Centrifugation Cell Counting
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