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Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation
 

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Article DOI: 10.3791/60324-v • 06:39 min • August 19th, 2020
August 19th, 2020 •
1, 1, 1, 1,2, 3, 4, 1
1Radboud Institute for Molecular Life Sciences, Department of Pathology, Radboud University Medical Center, 2Radboud Institute for Molecular Life Sciences, Amalia Children's Hospital, Department of Paediatric Nephrology, Radboud University Medical Center, 3Radboud Institute for Health Sciences, Department of Nephrology, Radboud University Medical Center, 4Radboud Institute for Molecular Life Sciences, Department of Nephrology, Radboud University Medical Center

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This article describes a method for culturing and analyzing glomerular parietal epithelial cell outgrowths of encapsulated glomeruli isolated from mouse kidney. This method can be used to study pathways involved in parietal epithelial cell proliferation and migration.

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Glomerular Outgrowth Ex Vivo Assay Pathways Parietal Epithelial Cell Activation Scar Tissue Development Kidney Glomerulus Cellular Processes Treatment Options Primary Cells Isolated Glomeruli Surgical Forceps Renal Capsules Culture Plate HBSS Petri Dish Scalpels Minced Kidney Pieces Metal Sieve Plunger Syringe Flow-through Serological Pipette Kidney Homogenate
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