Journal
/
Inmunología y contagio
/
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

DOI:
10.3791/60949-v

•

08:52 min

•

February 05, 2021

•

, , ,
1Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, 2Stony Brook Cancer Center, Stony Brook, NY, 3Department of Medicine,Weill Cornell Medicine, New York, NY

Capítulos

  • 00:05Introduction
  • 00:46Colonoscopy and Wound Induction
  • 02:46Wound Bed Visualization and Measurement
  • 04:37Wound Bed Collection
  • 06:02Histological Analysis Preparation
  • 07:45Results: Representative Wound Bed Visualization
  • 08:21Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we provide a detailed description of the procedure to induce colonoscopic-guided pinch biopsies in mice and track wound closure in real time. Additionally, methods for the preparation of tissues for histological, immunohistochemical and molecular analyses of the wound bed are provided.

Tags

Colonoscopic-guided Pinch BiopsyMiceColonic Wound HealingInflammatory Bowel Disease (IBD) PathogenesisEndoscopeBiopsy ForcepsAnesthesiaPBSVideo Recording

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Right Ventricular Systolic Pressure Measurements in Combination with Harvest of Lung and Immune Tissue Samples in Mice

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Author Spotlight: Evaluating Traditional Chinese Therapy for Ankylosing Spondylitis in Mice

Author Spotlight: Assessing Pain and Promoting Animal Welfare in Laboratory Animals Infected with Trypanosoma evansi

Read Article