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Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy
 

Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

Article DOI: 10.3791/61105-v • 08:56 min • May 8th, 2020
May 8th, 2020 •
1, 1, 1
1Department of Molecular Microbiology, Faculty of Biotechnology, University of Wroclaw

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Presented here is a protocol that describes monitoring of the complete life cycle of predatory bacterium Bdellovibrio bacteriovorus using time-lapse fluorescence microscopy in combination with an agarose pad and cell-imaging dishes.

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Live-cell Imaging Life Cycle Bacterial Predator Bdellovibrio Bacteriovorus Time-lapse Fluorescence Microscopy Stages Protocol Equalize Strains Bacterial Strains Pathogenic Strains B.bacteriovorus Culture Prey Culture Calcium Hippies Buffer Antibiotics Incubation Lysed Prey Cells Liquid Mounted Face Contrast Microscopy Multi-attack Phased B.bacteriovorus Cells E.coli Cell Bdelloplast Pore Size Filter Conical Tube Centrifuge Optical Density At 600 Nanometers
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