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JoVE Journal Neuroscience
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
 

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

Article DOI: 10.3791/61411-v • 06:53 min • February 12th, 2021
February 12th, 2021 •
1,2,3, 2,3
1School of Basic Medical Sciences, Xi'an Jiaotong University, 2Department of Cell Biology, Duke University, 3Department of Biochemistry, Duke University

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Here, we described a protocol to quantitatively study the assembly and structure of the axon initial segments (AIS) of hippocampal neurons that lack pre-assembled AIS due to the absence of a giant ankyrin-G.

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Primary Cultured Hippocampal Neurons Assembly Of Axon Initial Segments Hippocampal Neuron Structure Ankyrin-G Deficiency Dissection HBSS Medium Trypsin Digestion Pipetting Trituration Dissociated Neurons Plating Medium
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