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Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches
 

Microscopic Observation of Lymphocyte Dynamics in Rat Peyer's Patches

Article DOI: 10.3791/61568-v • 06:37 min • June 25th, 2020
June 25th, 2020 •
1,2, 1, 2, 1, 2, 1
1Department of internal medicine, National Defense Medical College, 2Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine

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Here, we describe a precise method for collecting thoracic duct lymphocytes and observing the migration of gut-tropic lymphocytes in rat Peyer’s patches for 3 hours using time-lapse photography. This technique can clarify how the dynamics of lymphocytes are affected under inflammatory conditions.

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Microscopic Observation Lymphocyte Dynamics Rat Peyer's Patches In Vivo Effects Of Tracks On Lymphocytes Kazuki Horiuchi Medical Doctor Cannulation Tube Hot Water Plastic Board Adhesive Tape Anesthetizing Wistar Rat Horizontal Incision Surgical Scissors Parietal Peritoneum Wet Gauze Retroperitoneal Organs Erector Muscles Adipose Tissue Connective Tissue Thoracic Duct Precision Tweezers Wet Cotton Swabs Ligature Abdominal Wall
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