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Genetics

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JoVE Journal Genetics
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
 

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Article DOI: 10.3791/61930-v • 08:30 min • February 9th, 2021
February 9th, 2021 •
1, 1, 2, 3, 2, 1,4
1Department of Biology, University of Florida, 2Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, 3Department of Radiation Oncology, Columbia University Medical Center, 4Center for Smell and Taste, University of Florida

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Many characteristics of insect eusociality rely on within-colony communication and division of labor. Genetic manipulation of key regulatory genes in ant embryos via microinjection and CRISPR-mediated mutagenesis provides insights into the nature of altruistic behavior in eusocial insects.

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Embryo Injections CRISPR-mediated Mutagenesis Ant Harpegnathos Saltator Genetically Modified Ants Gene Function In Communication And Behavior Genetic System Of Eusocial Insects Ant Hypernasal Cell Floor CRISPR-mediated Mutant Lines Graduate Student Ant Rearing Room Transparent Plastic Boxes Nest Boxes Plaster Floors Foam Block Designated Nest Region Live Crickets Plastic Nest Box Flooring Wash Bottle Disposal
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