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Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve
 

Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

Article DOI: 10.3791/62581-v • 07:34 min • August 5th, 2021
August 5th, 2021 •
1, 2, 2, 3, 3, 3, 2, 1
1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering, Ohio State University, 2Center for Regenerative Medicine, Nationwide Children’s Hospital, 3Thermo Fisher Scientific

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Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

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Surgery Sample Processing Correlative Imaging Murine Pulmonary Valve Heterogeneity Controlled Fixation High-resolution Images Anatomical Location Procedure Dr. Tai Yi Microsurgery Regenerative Medicine Autoclaving Tools Mouse Dissection Fine Scissors Microforceps Microvascular Clamps Clamp Applying Forceps Microneedle Holders Spring Scissors Retractors Euthanizing Mouse Dorsal Recumbent Position Thoracotomy Heart Exposure Adipose Tissue Removal Fascia Removal Saline Solution Perfusion Heart Removal Ventriculoatrial Junction Severing
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