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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
 

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Article DOI: 10.3791/62901-v • 06:00 min • September 20th, 2021
September 20th, 2021 •
*1,2, *1,2, 3, 4, 3, 3, 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally

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Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.

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Nuclei Isolation Adult Mouse Kidney Single-nucleus RNA-sequencing Gene Expression Data Complex Tissues Single-cell Level High-quality Data Protocol Dr. Janna Leiz Schmidt-Ott Lab Dissecting Plate Scalpel Razor Blade Microdissection Scissors Forceps Cortex Outer Medulla Inner Medulla RNA Stabilization Solution RNA Degradation Tissue Paper Minus 80 Degrees Celsius Polystyrene Petri Dish Nuclei Lysis Buffer 1 (NLB1) Mince Tissue
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