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亲和色谱法表达和纯化人基质金属蛋白酶-3
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Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
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亲和色谱法表达和纯化人基质金属蛋白酶-3

DOI:
10.3791/63263-v

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07:32 min

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March 30, 2022

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, ,
1Department of Chemical & Materials Engineering,University of Nevada, Reno

Capítulos

  • 00:04Introduction
  • 00:52MMP Expression
  • 03:46MMP Purification
  • 04:27MMP Refolding
  • 05:12MMP Activation
  • 05:46Results: Purification and Activation of Hisx6-Pro-MMP-3cd From BL21(DE3) and R2DP Cells
  • 06:38Conclusion

Summary

Traducción Automática

Traducción Automática

His-tag纯化，透析和活化用于提高细菌中可溶性活性基质金属蛋白酶-3催化结构域蛋白表达的产量。蛋白质级分通过SDS-PAGE凝胶进行分析。

Tags

Bacterial ExpressionRecombinant MMP-3Affinity ChromatographyE. ColiR2DP Cell StrainMMP InhibitorsProtein PurificationProtein FoldingPost-translational ProcessingIPTG InductionCell LysisInclusion Body SolubilizationSonicationCentrifugation

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