Journal
/
Biología del desarrollo
/
Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

Primary Cell Cultures to Study the Regeneration Potential of Murine Müller Glia after MicroRNA Treatment

DOI:
10.3791/63651-v

•

10:16 min

•

March 28, 2022

•

,
1Department of Biological and Vision Sciences, College of Optometry,The State University of New York

Capítulos

  • 00:05Introduction
  • 00:48Eyeball Cleaning, Retina Extraction, Transfer, and Washing
  • 03:06Retina Dissociation
  • 05:20Cell Passage to Remove Neuronal Survivors
  • 07:12Results: Tracking of Mller Glia Conversion into Progenitor Cells and Neuronal Identity Validation
  • 09:24Conclusion

Summary

Traducción Automática

Traducción Automática

Müller glia primary cultures obtained from mouse retinas represent a very robust and reliable tool to study the glial conversion into retinal progenitor cells after microRNA treatment. Single molecules or combinations can be tested before their subsequent application of in vivo approaches.

Tags

Artificial IntelligenceAI Writing AssistantsCopywritingContent GenerationText ProductionContent CreationAI-assisted Writing

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/63651?article_type=v

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
check_url/es/63651?article_type=v

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
check_url/es/63651?article_type=v

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
check_url/es/63651?article_type=v

Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
check_url/es/63651?article_type=v

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
check_url/es/63651?article_type=v

An Efficient Method to Obtain Dedifferentiated Fat Cells
check_url/es/63651?article_type=v

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
check_url/es/63651?article_type=v

Using Primary Neurosphere Cultures to Study Primary Cilia
check_url/es/63651?article_type=v

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
check_url/es/63651?article_type=v

Methods for the Study of Regeneration in Stentor

Read Article