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Biochemistry

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JoVE Journal Biochemistry
Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry
 

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Article DOI: 10.3791/63720-v • 09:20 min • April 11th, 2022
April 11th, 2022 •
1, 2, 2, 3, 1
1Department of Chemistry, University of Colorado, Denver, 2Department of Chemistry & Biochemistry, University of Denver, 3Analytical Resources Core Bioanalysis & Omics, Colorado State University

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MALDI-TOF was used to characterize fragments obtained from the reactivity between oxidized RNA and the exoribonuclease Xrn-1. The present protocol describes a methodology that can be applied to other processes involving RNA and/or DNA.

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Identification RNA Fragments Enzymatic Degradation MALDI-TOF Mass Spectrometry Methodology Characterization In Vitro Advantages Small Amounts Of Material Minimal Training DNA Proteins Biochemical Transformations Cross Information Chemical Modifications Biopolymer Interactions Reactivity MALDI Spectrometer Core Facilities Detection Limit UV-Vis Spectrophotometer PerkinElmer UV WinLab Icon Instrument Operation Control Window Peltier Temperature Controller Spectrophotometer Scan-Lambda 365
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