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Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

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Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

JoVE Revista
Biología

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JoVE Revista Biología

Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells

Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

DOI:

10.3791/65428-v

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02:28 min

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June 02, 2023

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Emily Jie-Ning Yang, Istvan R. Boldogh², Haojie Ji, Liza Pon², Theresa C. Swayne²

¹Department of Pathology and Cell Biology,Columbia University Irving Medical Center, ²Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center,Columbia University Irving Medical Center

Tags

Mitochondria Biosensor MtHyPer7 Hydrogen Peroxide Reactive Oxygen Species Live Cell Imaging Yeast Saccharomyces Cerevisiae Mitochondrial Function Mitochondrial Quality Control Cell Fitness Lifespan Ratiometric Genetically Encoded

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