A technique is presented for the in-vivo collection of interstitial fluid samples from pertinent tissue sites (here, experimentally inflamed skin) for the measurement of biochemicals mediating pain and inflammation.
Induction of experimental inflammation:
Catheter placement and sample collection:
The combined use of microdialysis techniques and multiplex immunoassay technology is a valuable real-time, in-vivo human bioassay providing insight into biochemical events in tissue of interest. Combined with behavioral tests such as pain tests, the method allows studying the complex interactions of biochemicals mediating pain and inflammation. The method is promising for 1) identifying tissue specific biomarkers mediating pain and inflammation, 2) providing mechanistic insight into the pathology of inflammatory and chronic pain conditions with peripheral tissue pathology, and 3) exploring analgesic/anti-hyperalgesic and anti-inflammatory actions of novel drug candidates and interventional therapies.
The authors have nothing to disclose.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
Microdialysis catheter | Tool | Dermal Dialysis | NA | |
Pump 22 | Tool | Harvard Apparatus | 552219 | |
Saalmann Multitester SBB LT 400 | Tool | Saalmann GmbH | NA | |
RM-12 IP-65 Radiometer | Tool | Dr. Grobel UV Elektronik GmbH | 82120000/81112100 | |
Complete Proteinase Inhibitor | Reagent | Roche Bioscience | 11836145001 | |
Microcent tube | Tool | E&K Scientific | 380150-X | |
Albumin 5% | Reagent | ZLB Behring | NA | |
Lactated Ringer | Reagent | Hospira Inc | NA | |
Xylocaine-MPF | Other | Astra Zeneca | NA | |
Syringe 1mL | Tool | Becton Dickinson Co | 309602 |