Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers (Video) | JoVE

Published: June 02, 2023

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10.3791/200065

Hugo Bibollet, Daniel F. Bennett, Martin F. Schneider, Erick O. Hernández-Ochoa

¹Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine

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Keywords: Cysteine-engineered Voltage-gated Ca2+ Channels Murine Isolated Skeletal Muscle Fibers CaV1.1 CDNA Construct EGFP-tagged Alpha Subunit Cytomegalovirus Promoter Site-directed Mutagenesis S4 Membrane Extracellular Interface GFP Observation Ionic Current Recording

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Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca²⁺ Channels in Murine Isolated Skeletal Muscle Fibers. J. Vis. Exp. (Pending Publication), e200065, doi:10.3791/200065 (2023).

Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers (Video) | JoVE

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