JoVE Journal > Medicine

Suspension-Trapping Sample Preparation of Human Tear Protein (Video) | JoVE

Published: December 01, 2023

doi:

Jimmy Sung-Hei Tse*¹, Ying-Hon Sze*^1,2, Jimmy Ka-Wai Cheung², King-Kit Li, Thomas Chuen Lam^2,3,4

¹Centre for Myopia Research, School of Optometry,The Hong Kong Polytechnic University, ²Centre for Eye and Vision Research (CEVR), ³Research Centre for SHARP Vision (RCSV),The Hong Kong Polytechnic University, ⁴Research Centre for Chinese Medicine Innovation (RCMI),The Hong Kong Polytechnic University

Tags

Keywords: Suspension-trapping Tear Protein Sample Preparation Dithiothreitol Iodoacetamide Phosphoric Acid Protein Binding Buffer TEAB Digestion Buffer Formic Acid Acetonitrile Gene Ontology Analysis Proteome Data Dependent Acquisition

Play Video

DOI

Citer Cet Article

Suspension-Trapping Sample Preparation of Human Tear Protein. J. Vis. Exp. (Pending Publication), e200490, doi:10.3791/200490 (2023).

View Video