RNA Fluorescence In Situ Hybridization to Localize the lncRNAs in Human Osteosarcoma Cells

RNA Fluorescence In Situ Hybridization to Localize the lncRNAs in Human Osteosarcoma Cells (Video) | JoVE

Published: June 16, 2023

doi:

10.3791/200526

Junli Chang*^1,2, Xiaoping Ma*^1,2, Xingyuan Sun², Chujie Zhou², Peng Zhao², Yongjun Wang², Yanping Yang²

¹Longhua Hospital,Shanghai University of Traditional Chinese Medicine, ²Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education

Tags

Keywords: RNA Fluorescence In Situ Hybridization LncRNA Localization 143B Osteosarcoma Cells Hybridization Buffer Probe Mix Cell Fixation Permeabilization Ethanol Dehydration Probe Hybridization Wash Buffers DAPI Staining Mounting

Play Video

PDF

DOI

Citer Cet Article

RNA Fluorescence In Situ Hybridization to Localize the lncRNAs in Human Osteosarcoma Cells. J. Vis. Exp. (Pending Publication), e200526, doi:10.3791/200526 (2023).

RNA Fluorescence In Situ Hybridization to Localize the lncRNAs in Human Osteosarcoma Cells (Video) | JoVE

Tags

Play Video

Citer Cet Article

View Video

✖

To prove you're not a robot, please enter the text in the image below