JoVE Journal > Chemistry

LC-TIMS-PASEF-ToF MS/MS Method for Proteolytic Histone Peptide Analysis

Published: January 12, 2024

doi:

Meiby Fernandez-Rojas, Cassandra N. Fuller, Lilian Valadares Tose, Matthew Willetts, Melvin A. Park, Natarajan V. Bhanu, Benjamin A. Garcia, Francisco Fernandez-Lima⁴

¹Department of Chemistry and Biochemistry,Florida International University, ²Bruker Daltonics Inc., ³Department of Biochemistry and Molecular Biophysics,Washington University School of Medicine, ⁴Biomolecular Sciences Institute,Florida International University

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LC-TIMS-PASEF-ToF MS/MS Proteolytic Histone Peptide Analysis HPLC C-18 Column TIMS ToF MS Nanoelectrospray Ionization Collision Energy LC Gradient MS-Digest Protein Prospector Post-translational Modifications Propionyl Acetyl HeLa S3 Cells

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DOI

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LC-TIMS-PASEF-ToF MS/MS Method for Proteolytic Histone Peptide Analysis. J. Vis. Exp. (Pending Publication), e200870, doi:10.3791/200870 (2024).

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