Source: Shankara Narayanan, J. S., et al. A Syngeneic Pancreatic Cancer Mouse Model to Study the Effects of Irreversible Electroporation. J. Vis. Exp. (2018).
This video describes the procedure for performing irreversible electroporation (IRE) for non-thermal ablation of pancreatic tumors in mice. Both subcutaneous (SQ) and orthotopic tumors can be successfully treated with IRE in a highly controlled setting, facilitating various studies post-procedure.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the jove veterinary review board.
1. IRE of SQ Tumors
2. IRE of Orthotopic Tumors
NOTE: IRE of orthotopic tumors involves a second survival surgery on the same mouse thus requiring special approval from local IACUC before beginning.
The authors have nothing to disclose.
ECM 830 square wave electroporator | Harvard Apparatus | BTX # 45-0002 ( 58018-004 ) | |
2 needle array electrode | Harvard Apparatus | 45-0167 | |
Safety foot switch | Harvard Apparatus | 45-0211 | |
Platinum Tweezer-trode | Harvard Apparatus | 45-0486 | |
Isoflurane | Sigma-Aldrich, Inc. | 792632 | |
Lacrilube | Fisher Scientific | 19090646 | |
Buprenorphine | Fisher Scientific | NC1292810 | |
D-luciferrin | Perkin Elmer | 122799 | |
IVIS Spectrum In Vivo Imaging System | Perkin Elmer | 124262 | |
Mouse strain C57BL/6J | The Jackson Laboratory | 000664/Black 6 | |
BD Precisionglide syringe needles | Sigma-Aldrich, Inc. | Z192406 | |
Alcohol Swab(70% isopropyl alcohol ) | BD | 326895 | |
Disposable Scalpels, Sterile | VWR | 21909 | |
Cotton Tipped Applicators | VWR | 89198 | |
Suture Needle, 45 cm, Size 6-0 | Harvard Apparatus | 72-3308 | |
Suture Needle, 45 cm, Size 4-0 | Harvard Apparatus | 72-3314 | |
Povidone-iodine 10% | BD | 29900-404 | |
Disposable Warming Pad | KENT SCIENTIFIC CORP | TP-3E | |
Mouse Hair Clipper | KENT SCIENTIFIC CORP | CL8787 | |
Surgical Drape | Harvard Apparatus | 59-7421 |