3D Technique to Visualize Mitochondrial Cristae in Pancreatic Cancer Cells

June 23, 2023

A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells (Video) | JoVE

Sample Preparation for the Three-Dimensional Reconstruction of Pancreatic Cancer Cell Mitochondria (Video) | JoVE

Image Acquisition and Three-Dimensional Reconstruction of Mitochondrial Cristae (Video) | JoVE

Yuwei Qi*¹, Yuhou Liu*¹, Ye Huang, Mengqing Xiong, Shumei You, Bo Wang, Mancang Gu³

¹School of Pharmaceutical Science of Zhejiang Chinese Medical University, ²Department of Pharmacy,Zhejiang Provincial Dermatology Hospital, ³Academy of Chinese Medical Sciences of Zhejiang Chinese Medical University

Summary

This protocol describes how to reconstruct mitochondrial cristae to achieve 3D imaging with high accuracy, high resolution, and high throughput.

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