Induction and Evaluation of Experimental Autoimmune Encephalomyelitis in Mouse

October 13, 2023

Creating a Versatile Experimental Autoimmune Encephalomyelitis Model Relevant for Both Male and Female Mice (Video) | JoVE

02:08 min

•

Preparation of MOG35-55 Emulsion for Immunization of Mouse (Video) | JoVE

01:01 min

•

Immunization of Mouse with MOG35-55 to Induce Experimental Autoimmune Encephalomyelitis (Video) | JoVE

00:56 min

•

Clinical Score and Motor Performance Evaluation of Mouse Induced with Experimental Autoimmune Encephalomyelitis (Video) | JoVE

01:52 min

•

Brigitta Bonaldo², Antonino Casile^2,3, Francesca Montarolo², Antonio Bertolotto⁴

¹Neuroscience Institute Cavalieri Ottolenghi (NICO), ²Department of Neuroscience “Rita Levi-Montalcini”,University of Turin, ³School of Pharmacy, Pharmacology Unit,University of Camerino, ⁴Koelliker Hospital

Summary

Experimental autoimmune encephalomyelitis is one of the most widely used murine models of multiple sclerosis. In the current protocol, C57BL/6J mice of both sexes are immunized with myelin oligodendrocyte glycoprotein peptide, resulting mainly in ascending paresis of the tail and limbs. Here we discuss the protocol of EAE induction and evaluation.