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Crystallization of ABC Transporter Membrane Proteins in Lipidic Bicelles

August 25, 2023
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Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion (Video) | JoVE

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1Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, 2Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

Summary

Traduction automatique

This protocol describes a setup for the crystallization of the sterol transporter ABCG5/G8. ABCG5/G8 is reconstituted into bicelles for hanging-drop crystallization. The protocol does not require specialized materials or substrates, making it accessible and easy to adapt in any laboratory for determining the protein structure through X-ray crystallography.

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