Enhancing EV Isolation for Precise Biofluid Analysis

October 27, 2023

Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery (Video) | JoVE

01:49 min

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Extracellular Vesicle (EV) Isolation from Human Urine Samples Using the EVtrap Approach (Video) | JoVE

01:54 min

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Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis (Video) | JoVE

02:56 min

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Proteome/Phosphoproteome Analysis of the Extracellular Vesicle (EV) Samples Using LC-MS/MS (Video) | JoVE

02:58 min

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Yi-Kai Liu, Zhuojun Luo, Anton Iliuk², Weiguo Andy Tao^2,3,4

¹Department of Biochemistry,Purdue University, ²Tymora Analytical Operations, ³Department of Chemistry,Purdue University, ⁴Purdue Institute for Cancer Research,Purdue University

Summary

The present protocol provides detailed descriptions for the efficient isolation of urinary extracellular vesicles utilizing functionalized magnetic beads. Moreover, it encompasses subsequent analyses, including western blotting, proteomics, and phosphoproteomics.