Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

December 22, 2023

細胞周期のG1期における一本鎖DNA病巣の可視化

遺伝毒性ストレスによるssDNA病巣形成を測定するためのG1同期細胞の調製

Qingyue Zhang², Marc A. Kerzhnerman², Nelson García-Vázquez^2,3, Gergely Rona^2,4,5

¹Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, ³Department of Cell Biology,NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute,NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

Summary

次のプロトコルはRPA2 immunofluorescent stainingに先行している細胞周期の同期を利用して細胞周期のG1段階のsingle-stranded DNAの焦点の検出を示す。

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