Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of <em>Arabidopsis</em> De-etiolation

Veronika Balakhonova; Nadiia Pushkarova; Jan Skalak; Tereza Dobisova; Zuzana Benedikty; Klara Panzarova; Martin Trtilek; Jan Hej&#225;tko

doi:10.3791/66087

High-Resolution Imaging of Chlorophyll Biosynthesis in Arabidopsis Seedling

January 12, 2024

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

01:43 min

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अरबिडोप्सिस थालियाना बीज सतह नसबंदी और विकास कक्ष में खेती

01:32 min

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क्लोरोफिल प्रतिदीप्ति माप और विश्लेषण iReenCAM प्रणाली का उपयोग कर Arabidopsis अंकुर में

01:41 min

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डेटा निष्कर्षण और Arabidopsis अंकुर में क्लोरोफिल प्रतिदीप्ति का विश्लेषण

03:09 min

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Veronika Balakhonova*^1,2, Nadiia Pushkarova*^1,3, Jan Skalak², Tereza Dobisova^2,4, Zuzana Benedikty, Klara Panzarova, Martin Trtilek, Jan Hejátko²

¹CEITEC – Central European Institute of Technology,Masaryk University, ²National Centre for Biomolecular Research, Faculty of Science,Masaryk University, ³Institute of Food Biotechnology and Genomics,National Academy of Sciences of Ukraine, ⁴Labdeers s.r.o, ⁵Photon Systems Instruments,Prumyslova

Summary

Here, we describe an advanced tool designed for chlorophyll biosynthesis monitoring during the early stages of Arabidopsis seedling de-etiolation. The novel methodology provides non-invasive real-time chlorophyll fluorescence imaging at high spatial and temporal resolution.