Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium

Kole H. Buckley; Keely A. Beyries; Sandra Ryeom; Sam S. Yoon; Bryson W. Katona

doi:10.3791/66094

Generating Human Gastric Organoids from Upper Endoscopy Biopsies

January 26, 2024

Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE

01:22 min

•

Isolating Single Cells from Upper Endoscopy Biopsies Derived from Gastric Patients for Organoid Generation (Video) | JoVE

02:18 min

•

Embedding Gastric Biopsy-Derived Single Cells in a Basement Membrane Matrix Dome for Organoid Generation (Video) | JoVE

02:03 min

•

Routine Passaging of Patient-Derived Gastric Organoids Through Fragmentation (Video) | JoVE

03:08 min

•

Kole H. Buckley, Keely A. Beyries, Sandra Ryeom, Sam S. Yoon, Bryson W. Katona

¹Division of Gastroenterology and Hepatology,University of Pennsylvania Perelman School of Medicine, ²Department of Surgery,Columbia University Irving Medical Center

Summary

Gastric patient-derived organoids find increasing use in research, yet formal protocols for generating human gastric organoids from single-cell digests with standardized seeding density are lacking. This protocol presents a detailed method for reliably creating gastric organoids from biopsy tissue obtained during upper endoscopy.