Rabbit Eye Dissection and Cryosectioning for Immunohistochemistry Studies

November 10, 2023

Whole Rabbit Eye의 고품질 냉동 절개 획득

Whole Rabbit Eye Dissection and Ocular Tissue Preparation for Histological Analysis

Ocular Tissue Embedding and Cryosectioning in Optimal Cutting Temperature Compound for Fluorescence Imaging

Erik A. Souverein, Aaron Nagiel^3,4, Ksenia Gnedeva⁶

¹Keck School of Medicine,University of Southern California, ²The Vision Center, Department of Surgery,Children’s Hospital Los Angeles, ³The Saban Research Institute,Children’s Hospital Los Angeles, ⁴USC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine,University of Southern California, ⁵Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research,University of Southern California, ⁶Tina and Rick Caruso Department of Otolaryngology-Head and Neck Surgery,University of Southern California

Summary

이 프로토콜은 전체 토끼 눈의 고품질 냉동 절개를 얻기 위한 신뢰할 수 있는 방법을 설명합니다. 토끼 눈 절제술, 고정, 포매 및 절편 절차에 대해 자세히 설명하며, 이는 큰 눈에 면역조직화학을 활용하는 모든 연구에 쉽게 적용할 수 있습니다.

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