Studying Adipose Endothelial Cell/Adipocyte Cross-Talk in Human Subcutaneous Adipose Tissue

Oliver  I. Brown; Katherine I. Bridge; Sam Straw; Natallia Makava; Jason Scragg; Sunti Limumpornpetch; Cheukyau Luk; Jessica Smith; Anna Skromna; Alexander F. Bruns; Piruthivi Sukumar; Lee D. Roberts; Richard Cubbon; Klaus K. Witte; Stephen Wheatcroft; Mark T. Kearney

doi:10.3791/66608

Human SATMVECs: Isolation and Adipocyte Cross-Talk Studies

April 05, 2024

Author Spotlight: Insights into Cardiometabolic Diseases with Subcutaneous Adipose Tissue Microvasculature Studies

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Oliver I. Brown, Katherine I. Bridge, Sam Straw, Natallia Makava, Jason Scragg, Sunti Limumpornpetch, Cheukyau Luk, Jessica Smith, Anna Skromna, Alexander F. Bruns, Piruthivi Sukumar, Lee D. Roberts, Richard Cubbon, Klaus K. Witte, Stephen Wheatcroft, Mark T. Kearney

¹Leeds Institute of Cardiovascular and Metabolic Medicine,University of Leeds

Summary

Here, we describe a protocol for isolating, culturing, and phenotyping microvascular endothelial cells from human subcutaneous adipose tissue (hSATMVECs). Additionally, we describe an experimental model of hSATMVEC-adipocyte cross-talk.

Human SATMVECs: Isolation and Adipocyte Cross-Talk Studies

Author Spotlight: Insights into Cardiometabolic Diseases with Subcutaneous Adipose Tissue Microvasculature Studies

Summary

Article