Long-Term Mouse Spinal Cord Organotypic Slice Culture as a Platform for Validating Cell Transplantation in Spinal Cord Injury

Francesca Merighi; Sara De Vincentiis; Marco Onorati; Vittoria Raffa

doi:10.3791/66704

Long-Term Engraftment of h-SC-NES Cells in Spinal Cord Organotypic Cultures

April 12, 2024

Author Spotlight: Long-Term Spinal Cord Slice Culture for Advancing Spinal Cord Regeneration Therapies

01:22 min

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Isolation and Slicing of Mouse Spinal Cord Lumbar Region to Generate Organotypic Slices for Regenerative Therapy Tests

03:00 min

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Transplantation of Human SC-NES Cells into Organotypic Slices for Validating Spinal Cord Regeneration Therapies

01:37 min

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Immunofluorescence Staining of Organotypic Slices with Stem Cell Transplants for Confocal Microscopy

01:50 min

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Francesca Merighi, Sara De Vincentiis, Marco Onorati, Vittoria Raffa

¹Department of Biology,University of Pisa

Summary

In this paper, we provide a reproducible method to generate and maintain long-term spinal cord organotypic slices transplanted with neural stem cells as an ex vivo model for testing cellular replacement therapies.