Isolating Immune Cells from Mouse Brain and Skull

Ran Zhang; Jin Zhang; Ata Ur Rehman; Lihong Dang; Xinyuan Yu; Wei Yang

doi:10.3791/66861

Obtaining Single-Cell Suspensions from Murine Brain and Skull Bone Marrow

July 26, 2024

Author Spotlight: Investigating the Complex Immune Response Following Brain Ischemia

02:10 min

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Ran Zhang, Jin Zhang, Ata Ur Rehman, Lihong Dang, Xinyuan Yu, Wei Yang

¹Multidisciplinary Brain Protection Program (MBPP), Department of Anesthesiology,Duke University Medical Center

Summary

To investigate the immune response to brain disorders, one common approach is to analyze changes in immune cells. Here, two simple and effective protocols are provided for isolating immune cells from murine brain tissue and skull bone marrow.

Obtaining Single-Cell Suspensions from Murine Brain and Skull Bone Marrow

Author Spotlight: Investigating the Complex Immune Response Following Brain Ischemia

Summary

Article