JOVE JOURNAL

Biochimie

Showing 493 - 504 in 724 videos.

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Hanna Axelsson², Helena Almqvist², Brinton Seashore-Ludlow³

¹Chemical Biology Consortium Sweden, Science for Life Laboratory,Karolinska Institutet, Solna, ²Department of Medical Biochemistry and Biophysics,Karolinska Institutet, Solna, ³Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet, Stockholm

Journal (Biochemistry)

Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay

Javad Alizadeh², Shahla Shojaei^2,3, Simone da Silva Rosa, Adel Rezaei Moghadam², Amir A. Zeki, Mohammad Hashemi, Marek J. Los^7,8, Joseph W. Gordon⁹, Saeid Ghavami^2,10

¹Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine,University of Manitoba, ²Biology of Breathing Theme, Children Hospital Research Institute of Manitoba,University of Manitoba, ³Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences,Isfahan University of Medical Sciences, ⁴Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine,Center for Comparative Respiratory Biology and Medicine, ⁵Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, ⁶Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec,Medical University of Silesia, ⁷Centre de biophysique moléculaire - UPR 4301,Centre national de la recherche scientifique (CNRS) CS80054, ⁸LinkoCare Life Sciences AB, ⁹College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences,University of Manitoba, ¹⁰Health Policy Research Center, Institute of Health,Shiraz University of Medical Sciences

Journal (Biochemistry)

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Tyler Mrozowich, Steffane McLennan, Michael Overduin⁴, Trushar R. Patel^3,4

¹Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry,University of Lethbridge, ²Department of Biochemistry,University of Alberta, ³Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary, ⁴DiscoveryLab, Faculty of Medicine & Dentistry,University of Alberta

Journal (Biochemistry)

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)

Graeden Winkelaar, Catharine Trieber, Jitendra Kumar, Michael Overduin

¹Department of Biochemistry,University of Alberta

Journal (Biochemistry)

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Smarajit Polley², De-Bin Huang, Tapan Biswas, Gourisankar Ghosh

¹Department of Chemistry & Biochemistry,University of California, San Diego, ²Department of Biophysics,Bose Institute

Journal (Biochemistry)

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Venkatasalam Shanmugabalaji, Véronique Douet, Birgit Agne, Felix Kessler

¹Laboratory of Plant Physiology,University of Neuchatel, ²Institut fur Biochemie und Biotechnologie,Martin-Luther-Universitat Halle-Wittenberg

Journal (Biochemistry)

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Anna Livia L. Matos*^1,3, David Grill*^1,3, Sergej Kudruk³, Nicole Heitzig³, Hans-Joachim Galla³, Volker Gerke³, Ursula Rescher³

¹Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation,University of Münster, ²Institute of Biochemistry,University of Münster, ³Cluster of Excellence 'Cells in Motion',University of Münster

Journal (Biochemistry)

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Takehiro Yamazaki*¹, Hiroaki Taniguchi*², Shoto Tsuji, Shiho Sato, Takahiro Kenmotsu, Kenichi Yoshikawa, Koichiro Sadakane

¹Faculty of Life and Medical Sciences,Doshisha University, ²The Institute of Genetics and Animal Breeding,Polish Academy of Sciences

Journal (Biochemistry)

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Jenny L. Carey, Olivia H. Cox, Fayaz Seifuddin, Leonard Marque, Kellie L.K. Tamashiro, Peter P. Zandi³, Gary S. Wand², Richard S. Lee

¹Departments of Psychiatry and Behavioral Sciences,Johns Hopkins School of Medicine, ²Department of Medicine,Johns Hopkins School of Medicine, ³Department of Mental Health,Johns Hopkins School of Public Health

Journal (Biochemistry)

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Ryan C. Oliver, Swe-Htet Naing, Kevin L. Weiss, Sai Venkatesh Pingali, Raquel L. Lieberman, Volker S. Urban

¹Neutron Scattering Division,Oak Ridge National Laboratory, ²School of Chemistry and Biochemistry,Georgia Institute of Technology

Journal (Biochemistry)

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Sofiia Karchugina, Jonathan Chernoff

¹Fox Chase Cancer Center

Journal (Biochemistry)

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Imen Bouchnak, Lucas Moyet, Daniel Salvi, Marcel Kuntz, Norbert Rolland

¹Université Grenoble Alpes, INRA, CNRS, CEA

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