JOVE JOURNAL

Neurosciences

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Journal (Neuroscience)

Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia

Claudia I Gutiérrez-Román², María E Meléndez Camargo, Cuauhtémoc C García Rojas, Miguel Jimenez Olvera, Sandra H Gutiérrez Román, Oscar Medina-Contreras

¹Epidemiology, Endocrinology & Nutrition,Mexico Children's Hospital, ²Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, ³Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service,Metropolitan Angeles Hospital

Journal (Neuroscience)

Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord

Claudia I Gutiérrez-Román², María E Meléndez Camargo, Cuauhtémoc C García Rojas, Miguel Jimenez Olvera, Sandra H Gutiérrez Román, Oscar Medina-Contreras

¹Epidemiology, Endocrinology & Nutrition,Mexico Children's Hospital, ²Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, ³Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service,Metropolitan Angeles Hospital

Journal (Neuroscience)

Optimizing Sample Preparation Process for Transmission Electron Microscopy of Neuromuscular Junctions in Drosophila Larvae

Gan Guangming², Sheng Qingyuan, Ou Yutong, Chen Mei, Zhang Chenchen

¹The School of Medicine,Southeast University, ²The Key Laboratory of Developmental Genes and Human Disease,Southeast University, ³The School of Life Science and Technology,Southeast University

Journal (Neuroscience)

Mouse Brain Dissection for Microglia Isolation

Morgan Towriss, Jennifer Kim, Annie Vogel Ciernia

¹Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, ²Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Journal (Neuroscience)

Mouse Brain Immune-Enriched Fragment Isolation for Histone PTM Quantification

Morgan Towriss, Jennifer Kim, Annie Vogel Ciernia

¹Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, ²Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Journal (Neuroscience)

Mouse Brain Enriched Immune Pellet Plate Staining Method for Histone Quantification

Morgan Towriss, Jennifer Kim, Annie Vogel Ciernia

¹Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, ²Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Journal (Neuroscience)

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

Morgan Towriss, Jennifer Kim, Annie Vogel Ciernia

¹Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, ²Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Journal (Neuroscience)

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

Morgan Towriss, Jennifer Kim, Annie Vogel Ciernia

¹Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, ²Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Journal (Neuroscience)

Mouse Leptomeninges Dissociation and Enzymatic Digestion for Efficient Culturing of Leptomeningeal Cells

Hong-Ji Deng, Kun Wu, Han-Fu Yu, Yong-Jin Zhang³, Yun-Cong Li, Chong Li, Fei Wang

¹Department of Neurosurgery,The First Affiliated Hospital of Kunming Medical University, ²Department of Clinical Laboratory,The First Affiliated Hospital of Kunming Medical University, ³Clinical Medical Research Center,The First Affiliated Hospital of Kunming Medical University

Journal (Neuroscience)

Establishing Primary In Vitro Culture of Leptomeningeal Lymphatic Endothelial Cells (LLECs)

Hong-Ji Deng, Kun Wu, Han-Fu Yu, Yong-Jin Zhang³, Yun-Cong Li, Chong Li, Fei Wang

¹Department of Neurosurgery,The First Affiliated Hospital of Kunming Medical University, ²Department of Clinical Laboratory,The First Affiliated Hospital of Kunming Medical University, ³Clinical Medical Research Center,The First Affiliated Hospital of Kunming Medical University

Journal (Neuroscience)

Harvest and Primary Culture of Leptomeningeal Lymphatic Endothelial Cells

Hong-Ji Deng, Kun Wu, Han-Fu Yu, Yong-Jin Zhang³, Yun-Cong Li, Chong Li, Fei Wang

¹Department of Neurosurgery,The First Affiliated Hospital of Kunming Medical University, ²Department of Clinical Laboratory,The First Affiliated Hospital of Kunming Medical University, ³Clinical Medical Research Center,The First Affiliated Hospital of Kunming Medical University

Journal (Neuroscience)

Microsurgical Creation of Giant Bifurcation Aneurysms in Rabbits for the Evaluation of Endovascular Devices

Branko Popadic^2,3, Florian Scheichel^2,3, Cornelia Pangratz-Daller^2,3, Roberto Plasenzotti, Camillo Sherif^2,3

¹Karl Landsteiner University of Health Sciences, ²Department of Neurosurgery,University Hospital St. Pölten, ³Cerebrovascular Research Group, Department of Neurosurgery,University Hospital St. Pölten, ⁴Division of Biomedical Research,Medical University of Vienna

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