All Protocols and Video Articles for scientific research | JoVE

Showing 217 - 228 of 477 videos for "Reconstitution"

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Journal (Biochemistry)

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Miroslava Stastna^2,3, Amandine Thomas, Juliana Germano, Somayeh Pourpirali, Jennifer E. Van Eyk², Roberta A. Gottlieb

¹The Smidt Heart Institute,Cedars-Sinai Medical Center, ²Advanced Clinical Biosystems Research Institute,Cedars-Sinai Medical Center, ³Institute of Analytical Chemistry of the Czech Academy of Sciences

Journal (Biochemistry)

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Keri L Colabroy, Katlyn Mayer

¹Department of Chemistry,Muhlenberg College

Journal (Neuroscience)

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Frédéric Knoflach, Maria-Clemencia Hernandez, Daniel Bertrand

¹Discovery Neuroscience, Pharma Research and Early Development,Roche Innovation Center Basel, ²HiQScreen Sàrl 6

Journal (Biochemistry)

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Francisco J. Sierra-Valdez, Richard A. Stein, Phanindra Velissety³, Valeria Vasquez, Julio F. Cordero-Morales

¹Department of Physiology,University of Tennessee Health Science Center, ²Department of Molecular Physiology and Biophysics,Vanderbilt University Medical Center, ³CuriRX, Inc.

Journal (Chemistry)

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Baharan Ali Doosti, Ann-Sofie Cans, Gavin D.M. Jeffries, Tatsiana Lobovkina

¹Department of Chemistry and Chemical Engineering,Chalmers University of Technology

Journal (Genetics)

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis

Jingxun Chen, David McSwiggen, Elçin Ünal

¹Department of Molecular and Cell Biology, Barker Hall,University of California, Berkeley, ²Department of Molecular and Cell Biology, Li Ka Shing Center,University of California, Berkeley

Journal (Neuroscience)

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Giulia Quattrocolo, Maria Isaac, Yajun Zhang, Timothy J. Petros

¹Kavli Institute for Systems Neuroscience and Centre for Neural Computation,Norwegian University of Science and Technology, ²Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health

Journal (Immunology and Infection)

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

Markus Horsthemke, Janine Wilden, Anne C. Bachg, Peter J. Hanley

¹Institut für Molekulare Zellbiologie

Journal (Biochemistry)

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Isabel M. Schopp², Julien Béthune²

¹Cluster of excellence CellNetworks,Heidelberg University, ²Heidelberg University Biochemistry Center (BZH)

Journal (Bioengineering)

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Frauke Hausburg*^1,2, Paula Müller*^1,2, Natalia Voronina*¹, Gustav Steinhoff², Robert David²

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,Rostock University Medical Center, ²Department Life, Light and Matter of the Interdisciplinary Faculty,Rostock University

Journal (Cancer Research)

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

Yu Okazawa², Kosuke Mizukoshi², Yu Koyama⁴, Shoki Okubo, Hiromitsu Komiyama, Yutaka Kojima, Michitoshi Goto, Sonoko Habu, Okio Hino, Kazuhiro Sakamoto, Akira Orimo

¹Department of Coloproctological Surgery,Juntendo University Faculty of Medicine, ²Department of Molecular Pathogenesis,Juntendo University Faculty of Medicine, ³Atopy Research Center,Juntendo University Faculty of Medicine, ⁴Department of Oral Pathobiological Science and Surgery,Tokyo Dental College, ⁵Department of Gastroenterology,Juntendo University Faculty of Medicine

Journal (Bioengineering)

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Chase A. Arbra*¹, Satish N. Nadig*¹, Sarah Grace Dennis, Sanket Pattanaik, Heather A. Bainbridge, J. Matthew Rhett, Stephen A. Fann, Carl Atkinson, Michael J. Yost

¹Department of Surgery,Medical University of South Carolina

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