All Protocols and Video Articles for scientific research | JoVE

Showing 277 - 288 of 553 videos for "Nucleophilic Enol"

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Journal (Genetics)

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Mithun Mitra², Ha Neul Lee^2,3, Hilary A. Coller^2,3

¹Department of Molecular, Cell and Developmental Biology,University of California, Los Angeles, ²Department of Biological Chemistry,David Geffen School of Medicine, ³Molecular Biology Institute Interdepartmental Program,University of California, Los Angeles

Journal (Bioengineering)

A Method for Determination and Simulation of Permeability and Diffusion in a 3D Tissue Model in a Membrane Insert System for Multi-well Plates

Hao-Hsiang Hsu, John-Kevin Kracht, Laura Elisabeth Harder, Kerstin Rudnik, Gerd Lindner, Katharina Schimek, Uwe Marx, Ralf Pörtner

¹Institute of Bioprocess and Biosystems Engineering,Hamburg University of Technology, ²Institute of Biotechnology, Department Medical Biotechnology,Technische Universität Berlin, ³TissUse GmbH

Journal (Genetics)

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Vid Leko², Smitha Sripathy, Robin L. Adrianse, Taylor Loe, Angela Park, Uyen Lao, Eric J. Foss, Marisa S. Bartolomei, Antonio Bedalov⁴

¹Clinical Research Division,Fred Hutchinson Cancer Research Center, ²Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, ³Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, ⁴Departments of Medicine and Biochemistry,University of Washington

Journal (Biochemistry)

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Eliza L. Lewis, Aaron M. Leconte

¹Department of Chemistry,W.M. Keck Science Department of Claremont Mckenna, Pitzer, and Scripps College

Journal (Biochemistry)

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Thomas Søndergaard Stenum, Michael A. Sørensen, Sine Lo Svenningsen

¹Department of Biology,University of Copenhagen

Journal (Chemistry)

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Andrew J. Francis, Marino J. E. Resendiz

¹Department of Chemistry,University of Colorado Denver

Journal (Bioengineering)

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

Mark Rustad, Allen Eastlund, Ryan Marshall, Paul Jardine, Vincent Noireaux

¹School of Physics and Astronomy,University of Minnesota, ²Department of Diagnostic and Biological Sciences and Institute for Molecular Virology,University of Minnesota

Journal (Biochemistry)

Determination of the Glycogen Content in Cyanobacteria

Alice De Porcellinis, Niels-Ulrik Frigaard, Yumiko Sakuragi

¹Carlsberg Research Laboratory, ²Department of Biology,University of Copenhagen, ³Copenhagen Plant Science Center, Department of Plant and Environmental Sciences,University of Copenhagen

Journal (Genetics)

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Benedetto Daniele Giaimo, Francesca Ferrante, Tilman Borggrefe

¹Institute of Biochemistry,University of Giessen

Journal (Genetics)

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae

Sebastian Grünberg, Gabriel E. Zentner

¹Basic Sciences Division,Fred Hutchinson Cancer Research Center, ²Department of Biology,Indiana University

Journal (Biology)

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Thea M. Edwards², Howard E. Morgan³, Coralia Balasca, Naveen K. Chalasani, Lauren Yam⁶, Alison M. Roark

¹Department of Biology,University of the South, ²School of Biological Sciences,Louisiana Tech University, ³School of Medicine,Louisiana State University Health Sciences Center, ⁴Department of Biology,Furman University, ⁵Department of Computer Science,Louisiana Tech University, ⁶Clemson University

Journal (Cancer Research)

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

Elisa Peranzoni*¹, Houcine Bougherara*¹, Sarah Barrin*¹, Audrey Mansuet-Lupo³, Marco Alifano, Diane Damotte³, Emmanuel Donnadieu

¹Institut Cochin, Inserm U1016-CNRS UMR8104,Université Paris Descartes, ²Department of Pathology, Paris Centre University Hospitals,Université Paris Descartes, ³INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center,University Pierre and Marie Curie, ⁴Department of Thoracic Surgery, Paris Centre University Hospitals,University Paris Descartes

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