All Protocols and Video Articles for scientific research | JoVE

Showing 85 - 96 of 477 videos for "Reconstitution"

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Journal (Immunology and Infection)

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Sean F. Gilmore*¹, Wei He*¹, Angela C. Evans, Delia F. Tifrea, Sukumar Pal, Brent Segelke, Sandra K. G. Peters, B. Dillon Vannest, Nicholas O. Fischer, Amy Rasley, Luis M. de la Maza, Matthew A. Coleman³

¹Physical and Life Sciences Directorate,Lawrence Livermore National Laboratory, ²Department of Pathology and Laboratory Medicine,University of California, ³School of Medicine, Radiation Oncology,University of California Davis

Journal (Biochemistry)

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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Chloe D. Truong², Dewight R. Williams, Mary Zhu, Joseph Che-Yen Wang, Po-Lin Chiu²

¹School of Molecular Sciences,Arizona State University, ²Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, ³Eyring Materials Center,Arizona State University, ⁴Department of Microbiology and Immunology,The Pennsylvania State University

Journal (Biochemistry)

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Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Linyu Zuo*¹, Jiawei Ding*¹, Zhi Qi

¹Center for Quantitative Biology, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies,Peking University

Journal (Biochemistry)

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Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Heidi L. Fresenius, Matthew L. Wohlever

¹Department of Chemistry & Biochemistry,University of Toledo

Journal (Bioengineering)

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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Jaime Lorenzo N. Dinglasan³, David T. Reeves³, Robert L. Hettich, Mitchel J. Doktycz

¹Graduate School of Genome Science & Technology,University of Tennessee Knoxville, ²Bredesen Center for Interdisciplinary Research,University of Tennessee Knoxville, ³Biosciences Division,Oak Ridge National Laboratory

Journal (Biochemistry)

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Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Laura K. Hillert-Richter, Inna N. Lavrik

¹Translational Inflammation Research, Center of Dynamic Systems,Otto von Guericke University Magdeburg

Journal (Bioengineering)

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Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors (GPCRs)

Ahmed Elbaradei*¹, Lucia Caterina Dalle Ore*¹, Noah Malmstadt

¹Mork Family Department of Chemical Engineering and Materials Science,University of Southern California

Journal (Biology)

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Neelima Boora*¹, Vibha Verma*¹, Ridhi Khurana, Gautam Gawande, Sanchi Bhimrajka, Komal Chaprana, Meenu Kapoor, Sanjay Kapoor

¹Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology,University of Delhi South Campus, ²University School of Biotechnology,Guru Gobind Singh Indraprastha University

Journal (Neuroscience)

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In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Swagata Dey, Anindya Ghosh-Roy

¹DBT-National Brain Research Centre

Journal (Neuroscience)

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Establishment of an Electrophysiological Platform for Modeling ALS with Regionally-Specific Human Pluripotent Stem Cell-Derived Astrocytes and Neurons

Arens Taga*¹, Christa W. Habela*¹, Alexandra Johns, Shiyu Liu, Mollie O'Brien, Nicholas J. Maragakis

¹Department of Neurology,Johns Hopkins University

Journal (Biochemistry)

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Phillip Wibisono, Jingru Sun

¹Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Journal (Bioengineering)

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Gabriela L. Santos², Tim Meyer², Malte Tiburcy², Alisa DeGrave², Wolfram-Hubertus Zimmermann^2,3,4,5, Susanne Lutz²

¹Institute of Pharmacology and Toxicology,University Medical Center Goettingen, ²DZHK (German Center for Cardiovascular Research) partner site, Goettingen, ³Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC),University of Goettingen, ⁴Center for Neurodegenerative Diseases (DZNE), ⁵Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)

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