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Showing 121 - 132 of 477 videos for "Reconstitution"

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Journal (Biochemistry)

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Collections de méthodes

Co-Translational Insertion of Membrane Proteins into Preformed Nanodiscs

Roman Levin, Zoe Koeck, Volker Dötsch, Frank Bernhard

¹Institute of Biophysical Chemistry,Goethe University Frankfurt

Journal (Biochemistry)

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Eduardo Hilario, Li Fan, Leonard J. Mueller, Michael F. Dunn

¹Department of Chemistry,University of California-Riverside, ²Department of Biochemistry,University of California-Riverside

Journal (Biochemistry)

Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

Satish Bodakuntla*^1,2, A.S. Jijumon*^1,2, Carsten Janke², Maria M. Magiera²

¹PSL Research University, CNRS UMR3348,Institut Curie, ²Université Paris-Saclay, CNRS UMR3348,Université Paris Sud

Journal (Bioengineering)

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Irini M. Dijkhoff, Benedetta Petracca², Roxane Prieux, Giuseppe Valacchi⁴, Barbara Rothen-Rutishauser, Marc Eeman

¹Adolphe Merkle Institute,University of Fribourg, ²Dow Silicones Belgium SRL, ³Department of Biomedical and Specialist Surgical Sciences,University of Ferrara, ⁴Department of Animal Sciences, Plants for Human Health Institute,North Carolina State University

Journal (Biochemistry)

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Alexander C. Y. Foo, Peter M. Thompson, Geoffrey A. Mueller

¹Nuclear Magnetic Resonance Group,National Institute of Environmental Health Sciences

Journal (Bioengineering)

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Bingchen Che*¹, Jie Zhu*¹, Dan Sun, Xiaoqiang Feng, Ce Zhang

¹State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology,Northwest University

Journal (Biology)

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Wojciech Wiertelak, Mariusz Olczak, Dorota Maszczak-Seneczko

¹Laboratory of Biochemistry, Faculty of Biotechnology,University of Wroclaw

Journal (Neuroscience)

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Sovannarath Pong^2,3, Paulo Lizano^2,3,4, Rakesh Karmacharya^3,4,5

¹Center for Genomic Medicine,Massachusetts General Hospital, ²Department of Psychiatry,Beth Israel Deaconess Medical Center, ³Chemical Biology and Therapeutic Science Program,Broad Institute of MIT and Harvard, ⁴Department of Psychiatry,Harvard Medical School, ⁵Schizophrenia and Bipolar Disorder Program,McLean Hospital

Journal (Biochemistry)

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

Yifan Ge, Sivakumar Boopathy, Adam Smith, Luke H. Chao³

¹Department of Molecular Biology,Massachusetts General Hospital, ²Department of Chemistry,University of Akron, ³Department of Genetics,Harvard Medical School

Journal (Bioengineering)

Manipulation of Single Neural Stem Cells and Neurons in Brain Slices using Robotic Microinjection

Gabriella Shull*^1,2, Christiane Haffner*³, Wieland B. Huttner, Elena Taverna⁴, Suhasa B. Kodandaramaiah^5,6

¹Department of Biomedical Engineering,University of Minnesota, ²Department of Biomedical Engineering,Duke University, ³Max Planck Institute of Molecular Cell Biology and Genetics, ⁴Max Planck Institute for Evolutionary Anthropology, ⁵Department of Mechanical Engineering,University of Minnesota, ⁶Graduate Program in Neuroscience,University of Minnesota

Journal (Biochemistry)

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Collections de méthodes

Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

Sandeep K. Misra, Joshua S. Sharp^2,3

¹Department of Biomolecular Sciences,University of Mississippi, ²Department of Chemistry and Biochemistry,University of Mississippi, ³GenNext Technologies, Inc.

Journal (Bioengineering)

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Latifa Bouzhir*¹, Emilie Gontran*¹, Lorena Loarca, Mauricette Collado-Hilly, Pascale Dupuis-Williams²

¹Physiopathogénèse et traitement des maladies du foie,Université Paris-Saclay, Inserm U1193, ²ESPCI Paris, Université PSL

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