В этой статье описываются высоким временным и пространственным разрешением технику оптически образ потенциала действия движения на поверхности Langendorff-перфузии сердца крысы использованием потенциометрического красителя (ди-8-ANEPPS).
Удаление сердца от анестезии крыс должна быть выполнена быстро, чтобы избежать ишемии миокарда. Если ишемия или недостаточное коронарной перфузии происходит, сердце, скорее всего, развиваться аритмий и может стать инфаркта. Кроме того, эти сердца покажет недостаточной флуоресценции в…
The authors have nothing to disclose.
Эта работа проводится при поддержке грантов от Национального института здоровья (HL068915; HL088206) и взносов в Фонд сердечной проводимости при Детской больнице Бостона.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
CardioCMOS-SM128f | Equipment | RedShirt Imaging, Decatur, GA 30030 USA | ||
CardioPlex Software | Equipment | RedShirt Imaging, Decatur, GA 30030 USA | ||
LUXEON LED Light Source 460-490 nm | Equipment | Lumileds Lighting, US, LLC, San Jose, CA 95131 USA | LXHL-PB02 | |
ECG Amplifier Type 689 Hugo Sachs Elektronik | Equipment | Harvard Apparatus, Holliston, MA 01746 USA | 730149 | |
Dichroic Mirror 505 nm | Equipment | Semrock, Rochester, NY 14624 USA | FF505-SDi01-25×36 | |
Emission Filter 605 nm Long Pass | Equipment | SciMedia, Costa Mesa, CA 92626 USA | ||
THT Sideways | Equipment | SciMedia, Costa Mesa, CA 92626 USA | 25 BM-8 | |
Mini Ball Joint Holder | Equipment | Harvard Apparatus, Holliston, MA 01746 USA | BS4 73-0177 | |
Small Stimulation Electrode Set | Equipment | Harvard Apparatus, Holliston, MA 01746 USA | BS4 73-0160 | |
BM-6 Benchtop Vibration Isolation Platform | Equipment | Technology Inc., Inglewood, CA 90301 | 25 BM-6 | |
Monopolar ECG Electrode | Equipment | Harvard Apparatus, Holliston, MA 01746 USA | BS4 73-0200 | |
Roller Pump SCI 400 | Equipment | Watson-Marlow Bredel Inc., Wilmington, MA 01887 USA | 401U/D1 | |
Roller Pump MasterFlex Easy Load II | Equipment | Cole Parmer, Vernon Hills, Illinois 60061 USA | Model 77201-60 | |
Tubing Marprene #14 | Equipment | Watson-Marlow Bredel Inc., Wilmington, MA 01887 USA | 902.0016.016 | |
MasterFlex Tubing | Equipment | PharMed, Westlake, OH 44145 USA | 06485-25 | |
S48 Square Pulse Stimulator | Equipment | Grass Technologies, West Warwick, RI 02893 USA | Model S48 | |
SIU5 RF TRANSFORMER ISOLATION UNIT | Equipment | Grass Technologies, West Warwick, RI 02893 USA | Model SIU5 | |
5 Liter Water Jacketed Reservoir | Equipment | Radnoti Glass Technology Inc., Monrovia CA 91016 USA | 120142-5 | |
2 Liter Water Jacketed Reservoir | Equipment | Radnoti Glass Technology Inc., Monrovia CA 91016 USA | 120142-2 | |
0.5 Liter Water Jacketed Reservoir | Equipment | Radnoti Glass Technology Inc., Monrovia CA 91016 USA | 120142-0 | |
0.25 Liter Water Jacketed Reservoir | Equipment | Radnoti Glass Technology Inc., Monrovia CA 91016 USA | 120142-025 | |
10 ml Heating Coil | Equipment | Radnoti Glass Technology Inc., Monrovia CA 91016 USA | 158822 | |
Compliance Bubble Trap | Equipment | Radnoti Glass Technology Inc., Monrovia CA 91016 USA | 130149 | |
Luer Disconnect Cannula | Equipment | Harvard Apparatus, Holliston, MA 01746 USA | 72-1444 | |
3-Way stopcock, FLL to MLT, No Port Covers | Equipment | Harvard Apparatus, Holliston, MA 01746 USA | BS4 72-2630 | |
Thermocouple Thermometer | Equipment | Cole Parmer, Vernon Hills, Illinois 60061 USA | WU-91100-40 | |
Ultra Fine IT-Series Flexible Microprobe | Equipment | PhysiTemp Instruments Inc., Clifton, NJ 07013 USA | IT-24P | |
Oscilloscope Tektronix TDS 1002 | Equipment | Tektronix Inc., Beaverton, OR 97005 USA | TDS 1002B | |
2,3-Butanedione monoxime | Reagent | Sigma, St. Louis, MO 63132 USA | B0753 | |
Ketamine HCl | Reagent | Hospira Inc., Lake Forest, IL 60045 USA | RL-0065 | |
Xylazine | Reagent | Lloyd Inc., Iowa 51601 USA | LB15705A | |
E-TOXA-CLEAN® | Reagent | Sigma, St. Louis, MO 63132 USA | E9029 | |
Di-8-ANEPPS | Reagent | Invitrogen, Carlsbad, CA 92008 USA | D-3167 |