MYTH allows the sensitive detection of transient and stable interactions between proteins that are expressed in the model organism Saccharomyces cerevisiae. It has been successfully applied to study exogenous and yeast integral membrane proteins in order to identify their interacting partners in a high throughput manner.
MYTH is the first high throughput system that allows the identification of interactions between full-length membrane proteins and cytosolic or membrane-bound partners. It has been used to study membrane protein from a range of organisms [3-7]. There are, however, specific details that may need to be scrutinized to ensure the protein-of-interest is amenable to study with MYTH.
Many membrane-bound proteins are directed to the plasma membrane via a signal sequence that is subsequently cleaved to…
The authors have nothing to disclose.
We would like to thank Dawn Edmonds for a critical reading of this manuscript. The Stagljar lab is supported by funds from the Canadian Foundation for Innovation (CFI), the Canadian Institute for Health Research (CIHR), the Heart and Stroke Foundation, the Canadian Cancer Society, and Novartis.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
Polyethenlene Glycol (PEG3350) | Bioshop | PEG335 | ||
Lithium Acetate Bihydrate | Bioshop | LIA001 | ||
X-Gal (5-Bromo-4-Chloro-3-Indolyl-b-D-galactopyranoside) | Bioshop | XGA001 | ||
N`,N-dimethyl formamide | Bioshop | DMF 451 | ||
3-amino-1,2,4-triazole (3-AT) | Bioshop | ATT124 | ||
Sodium phosphate dibasic | Bioshop | SPD307 | ||
Sodium phosphate monobasic | FisherBiotech | BP329-500 | ||
Salmon Sperm DNA | VWR | CA80601-120 | ||
D-Glucose | Bioshop | GLU501 | ||
LB Broth LENOX | Bioshop | LBL405 | ||
Yeast Nitrogen Base | Bioshop | YNB406 | ||
Yeast Extract | Bioshop | YEX401 | ||
Peptone | Beckton Dickinson | 211677 | ||
Bio-Tryptone | Bioshop | TRP402 | ||
Adenine Sulphate | Bioshop | ADS201 | ||
L-Uracil | Bioshop | URA241 | ||
L-Threonine | Bioshop | THR002 | ||
L-Histidine | Bioshop | HIS200 | ||
L-Methionine | Bioshop | MET222 | ||
L-Valine | Bioshop | VAL201 | ||
L-Phenylalanine | Bioshop | PHA302 | ||
L-Isoleucine | Bioshop | ISO910 | ||
L-Tyrosine | Bioshop | TYR333 | ||
L-Leucine | Bioshop | LEU222 | ||
L-Arginine | Bioshop | ARG006 | ||
L-Tryptophane | FisherBiotech | BP395-100 | ||
L-Lysine | Bioshop | LYS101 | ||
L-Alanine | FisherBiotech | BP369-100 | ||
Agar | Bioshop | AGR001 | ||
Soda Lime Galss Beads | BioSpec Product | 11079105 | ||
Sodium Chloride | Bioshop | SLD002 |