在小鼠分离的骨骼肌纤维中转染半胱氨酸工程电压门控CA²⁺ 通道的构建设计

Published: June 02, 2023

doi:

10.3791/200065

Hugo Bibollet, Daniel F. Bennett, Martin F. Schneider, Erick O. Hernández-Ochoa

¹Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine

Tags

Keywords: Cysteine-engineered Voltage-gated Ca2+ Channels Murine Isolated Skeletal Muscle Fibers CaV1.1 CDNA Construct EGFP-tagged Alpha Subunit Cytomegalovirus Promoter Site-directed Mutagenesis S4 Membrane Extracellular Interface GFP Observation Ionic Current Recording

Play Video

PDF

DOI

Citer Cet Article

Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca²⁺ Channels in Murine Isolated Skeletal Muscle Fibers. J. Vis. Exp. (Pending Publication), e200065, doi:10.3791/200065 (2023).

在小鼠分离的骨骼肌纤维中转染半胱氨酸工程电压门控CA2+ 通道的构建设计

Tags

Play Video

Citer Cet Article

View Video

✖

To prove you're not a robot, please enter the text in the image below

在小鼠分离的骨骼肌纤维中转染半胱氨酸工程电压门控CA²⁺ 通道的构建设计