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JoVE Journal > Immunology and Infection
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Immunologie et infection
Gastric Fluid Sampling, DNA Extraction, and qPCR for
H. pylori
Detection and its Antibiotic Resistance
Published: July 28, 2023
doi:
10.3791/200247
Liang Wang*
1,2,3
,
Jin-Xin Lai*
1
,
Yu-Ting Si*
1,4
,
Xu-Xia Cui
4
,
Zeeshan Umar
5
,
Xiao-Jun Ru
,
Xin-Yu Zhang
,
Zheng-Kang Li
,
Alfred Chin Yen Tay
6,7,8
,
Barry J. Marshall
6,7,8
,
Guang-Hua Li
,
Bing Gu
1
Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences)
,
Southern Medical University
,
2
Division of Microbiology and Immunology, School of Biomedical Sciences
,
The University of Western Australia
,
3
Center for Precision Health, School of Medical and Health Sciences
,
Edith Cowan University
,
4
Medical Technology School of Xuzhou Medical University
,
5
Marshall Laboratory of Biomedical Engineering, School of Medicine
,
Shenzhen University
,
6
The Marshall Centre for Infectious Diseases Research and Training
,
The University of Western Australia
,
7
Marshall International Digestive Diseases Hospital
,
Zhengzhou University
,
8
Marshall Medical Research Center
,
Fifth Affiliated Hospital of Zhengzhou University
Tags
Gastric Fluid Sampling
DNA Extraction
QPCR
H. Pylori Detection
Antibiotic Resistance
String Test Kit
TSE Sample Preservation
Nucleic Acid Extractor
PCR Reaction Mixture
23S RRNA
GyrA Gene Mutation
Clarithromycin Resistance
Levofloxacin Resistance
check_url/fr/200247?article_type=t
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Gastric Fluid Sampling, DNA Extraction, and qPCR for
H. pylori
Detection and its Antibiotic Resistance.
J. Vis. Exp.
(Pending Publication), e200247, doi:10.3791/200247 (2023).
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