Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API)

doi:10.3791/200724

JoVE Journal > Biology

Biologie

Klargjøring av FFPE DNA- og RNA-prøver for sekvensering ved bruk av et automatisert renseinstrument (API)

Published: September 08, 2023

doi:

10.3791/200724

Christophe Bontoux^2,3,4,5, Virginie Lespinet-Fabre^2,3,4, Olivier Bordone^2,3,4, Virginie Tanga^2,3,4, Maryline Allegra^2,3,4, Myriam Salah^2,3,4, Salomé Lalvée^2,3,4, Samantha Goffinet^2,3,4, Jonathan Benzaquen^4,5,6, Charles-Hugo Marquette^4,5,6, Marius Ilié^2,3,4,5, Véronique Hofman^2,3,4,5, Paul Hofman^2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge,Université Côte d’Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d’Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur

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Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API). J. Vis. Exp. (Pending Publication), e200724, doi:10.3791/200724 (2023).

Klargjøring av FFPE DNA- og RNA-prøver for sekvensering ved bruk av et automatisert renseinstrument (API)

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