C. elegans Gonad Extrusion: A Rapid Dissection Technique
Published: April 30, 2023
Abstract
Source: Gervaise, A. L. and Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (2016).
This video describes a method to dissect gonads from adult C. elegans hermaphrodites, resulting in tissues that are suitable for immunostaining and fluorescent imaging.
Protocol
This protocol is an excerpt from Gervaise and Arur, Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (2016). 1. Dissection of Adult Worms for Obtaining Gonads Pick 100 – 150 WT (N2) or desired genotype of worms at the desired and specific developmental stage (L1, L2, …
Materials
| Flat bottom glass watch dish | Agar Scientific | AGL4161 | We use glass because dissected gonads often stick to plastic |
| 25 G needles | BD PrecisionGlide | 305122 | |
| Syringes (could be 1 or 5 mL) | BD Syringes | 1 mL: BD 309659 | |
| Microscope slides (25 x 75 x 1.0 mm) | Fisherbrand | 12-550-343 | |
| Clinical bench top centrifuge | |||
| *M9 solution | |||
| Levamisole | Sigma | L9756 | |
| *M9 Buffer Recipe | 3 g KH2PO4, 6 g Na2HPO4, 5 g NaCl, 1 mL 1 M MgSO4, H2O to 1 L. |
Citer Cet Article
C. elegans Gonad Extrusion: A Rapid Dissection Technique. J. Vis. Exp. (Pending Publication), e20131, doi: (2023).